Предложен способ определения трициклического антидепрессанта амитриптилина амперометрическими иммуносенсорами на основе печатных графитовых электродов, модифицированных электрохимически осажденными наночастицами гидроксида никеля и углеродными наноматериалами в сочетании с производными каликс[4]аренов, в урине человека. Рассмотрены различные комбинации углеродных материалов (углеродных нанотрубок, бумаги из углеродных нанотрубок), каликс[4]аренов и их производных (тиакаликс[4]аренов, аминотиакаликс[4]аренов) и электрохимически осажденных наночастиц гидроксида никеля. В качестве аналитического сигнала выбран ток пика окисления наночастиц гидроксида никеля, потенциал которого за счет добавления к композитному модификатору биораспознающего компонента (антител) смещается на 220 мВ. Рассмотрены варианты иммуносенсоров, отличающиеся составом углеродных материалов. Варьирование состава композитного модификатора оказывает влияние на изменение тока пика окисления наночастиц гидроксида никеля в присутствии амитриптилина, что отражается на аналитических возможностях иммуносенсоров. Лучшие аналитические характеристики, т. е. диапазон определяемых концентраций 1×10^-9 - 1×10^-5 М, предел определения 5×10^-10 М (при S_r=0.33) получены для амперометрического иммуносенсора, модифицированного бумагой из углеродных нанотрубок (массовый эквивалент 0.9 по KMnO₄, неотожжённая в печи) с аминотиакаликс[4]ареном и электрохимически осажденными наночастицами гидроксида никеля. Разработана методика определения амитриптилина амперометрическими иммуносенсорами на основе композитных материалов в урине человека. Относительное стандартное отклонение при определении амитриптилина не превышает 0.075.

Ключевые слова: иммуносенсор, наночастицы гидроксида никеля, углеродные нанотрубки, каликс[4]арены, бумага из углеродных нанотрубок, амитриптилин

иммуносенсор; наночастицы гидроксида никеля; углеродные нанотрубки; каликс[4]арены; бумага из углеродных нанотрубок; амитриптилин

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