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Одновременное определение 14 родентицидов в патологическом материале методом ВЭЖХ-МС/МС после очистки методом твердофазной экстракции

E. D. Usanova, E. S. Osinova, M. Yu. Parfenov, V. V. Tolmacheva, A. O. Melekhin, V. V. Tishchenko

Аннотация


Предложен способ определения родентицидов в патологическом материале методом высокоэффективной жидкостной хроматографии-тандемной масс-спектрометрии. При разработке методики были сравнены различные способы пробоподготовки. В результате сравнения был выбран способ, включающий себя жидкостную экстракцию с помощью метанола и дальнейшую очистку экстракта методом твердофазной экстракции на сорбенте C18. Разработанная методика валидирована, пределы определения составили 2-5 мкг/кг. Относительное стандартное отклонение результатов анализа не превышает 20 %. Матричные эффекты при определении не превышали 25 %.

Ключевые слова: родентициды, патологический материал, ВЭЖХ-МС/МС, твердофазная экстракция, экстракционное выделение из мышечной ткани


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DOI: https://doi.org/10.15826/analitika.2025.29.4.005

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