Одновременное определение 12 аминогликозидов в растительной продукции методом ВЭЖХ-МС/МС после извлечения методом твердофазной экстракции
Аннотация
Разработан упрощенный и эффективный вариант пробоподготовки в сочетании с высокоэффективной жидкостной хроматографией-тандемной масс-спектрометрией (ВЭЖХ-МС/МС) для определения двенадцати аминогликозидных антибиотиков-пестицидов: амикацина, апрамицина, валидамицина, гентамицина, гигромицина, дигидрострептомицина, канамицина, касугамицина, неомицина, паромомицина, стрептомицина, чжуншэнмицина в матрицах с высоким содержанием крахмала (рис), высокой кислотностью (лимон), высоким содержанием жира (авокадо) и высоким содержанием воды (огурец). Сущность метода заключается в экстракции соединений специфичным буферным раствором, состоящим из ацетата аммония, ЭДТА, хлорида натрия и трихлоруксусной кислоты и последующем извлечении аналитов из экстрактов на картриджах HLB. Результаты валидации оказались удовлетворительными: для всех целевых соединений коэффициенты корреляции для градуировочных зависимостей (R2) превышали 0,99 в линейном диапазоне в четырех матрицах. Пределы обнаружения (LOD) составляли 3–15 мкг/кг, а пределы количественного определения (LOQ) 10–50 мкг/кг. Междневные и внутридневные относительные стандартные отклонения (RSD) были менее 20 %.
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DOI: https://doi.org/10.15826/analitika.2026.30.1.005
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