Cover Image

Testing the antigenotoxicity and anticytotoxicity properties of Prunella Grandiflora L. extract using the example of Drosophila Melanogaster

Ngonidzaishe Magombe, Viktoria V. Kostenko, Olga N. Antosyuk, Elizaveta V. Bolotnik


Using the Drosophila melanogaster model object, the presence of protective properties in 10% of Prunella grandiflora L. (Lamiaceae) extract was determined in relation to the toxic and cytotoxic effects of the antitumor drug etoposide in two concentrations of 800 and 8000 µg/kg of nutrient medium. The grass P. grandiflora was collected in the flowering phase in Sverdlovsk region (N 56°09'22.0'', E 058°32'19.6'') in 2021. No genotoxic manifestations of the extract were found. Comparative characteristics of wing parameters under the influence of etoposide at a dose of 800 and 8000 mg showed significant differences in linear and two-dimensional parameters of the wing.


extract; prunella; etoposide; morphometry; antigenotoxic

Full Text:



Huang M, Wang Y, You M. Anti-tumor properties of Prunella Vulgaris. Springer International Publishing, AG; 2015, 401–419 p.

Xavier CP, Lima CF, Fernandes-Ferreira M, Pereira-Wilson C. Salvia fruticosa, Salvia officinalis, and rosmarinic acid induce apoptosis and inhibit proliferation of human colorectal cell lines: the role in MAPK/ERK Pathway. Nutr Cancer. 2009;61(4):564–571. doi:10.1080/01635580802710733

Rajendra NP, Karthikeyan A, Karthikeyan S, Reddy BV. Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell line. Mol Cell Biochem. 2011;349(1–2):11–19. doi:10.1007/s11010-010-0655-7

Budantsev AL. Plant resources of Russia: wild flowering plants, their component composition and biological activity: Botanical Institute named after V. L. Komarova. St. Petersburg; 2008). 236–239 p.

Mukhopadhyay A, Vij Shubha S, Tyagi A. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proc Nat Acad Sci USA. 2004;101:6309–6314. doi:10.1073/pnas.0401572101

Plyusnina E, Shaposhnikov M, Moskalev A. Increase of Drosophila melanogaster lifespan due to D-GADD45 overexpression in the nervous system. Biogerontol. 2010;12(3):211–226. doi:10.1007/s10522-010-9311-6

Carmona ER, Guecheva TN, Creus A, Marcos R. Proposal of an in vivo comet assay using haemocytes of Drosophila melanogaster. Environ Mol Mutagen. 2011;52(2):165–169. doi:10.1002/em.20604

Irving P, Ubeda J, Doucet D, Troxler L, Lagueux M, Zachary D, Hoffmann J, Hetru C, Meister M. New insights into Drosophila larval haemocyte functions through genome-wide analysis. Cell Microbiol. 2005;7:335–350. doi:10.1111/j.1462-5822.2004.00462.x

Sharma A, Mishra M, Ram KR, Kumar R, Abdin MZ, Chowdhuri DK. Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. Chemosphere. 2011;82(3):370–376. doi:10.1016/j.chemosphere.2010.10.002

Delia D, Mizutani S. The DNA damage response pathway in normal hematopoiesis and malignancies. Int J Hematol. 2017;106(3):328–334. doi:10.1007/s12185-017-2300-7

Roos WP, Kaina B. DNA damage-induced cell death: from specific DNA lesions to the DNA damage response and apoptosis. Cancer Lett. 2013;332(2):237–248. doi:10.1016/j.canlet.2012.01.007

Goldstein M, Kastan MB. The DNA damage response: implications for tumor responses to radiation and chemotherapy. Annu Rev Med. 2015;66:129–143. doi:10.1146/annurev-med-081313-121208

Magombe N, Antosyuk ON, Bolotnik EV. Izuchenie protektornyh svojstv ekstrakta Prunella grandiflora L. otnositel'no vozdejstviya protivoopuholevogo preparata etopozid na primere Drosophila melanogaster. In: Abstracts of the Openbio VIII Mezhdunarodnaya nauchno-prakticheskaya konferenciya molodyh uchenyh: biofizikov, biotekhnologov, molekulyarnyh biologov i virusologov, 2021 Oct 5-8, Novosibirsk, Russia. p. 35–36.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Copyright (c) 2022 Ngonidzaishe Magombe, Viktoria V. Kostenko, Olga N. Antosyuk, Elizaveta V. Bolotnik

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Scopus logo WorldCat logo DOAJ logo CAS logo BASE logo eLibrary logo

Chimica Techno Acta, 2014-2023
ISSN 2411-1414 (Online)
Copyright Notice