T. A. Denisenko, A. B. Vishnikin, L. P. Tsiganok


The comparative investigation of a reaction between 18-molybdophosphate heteropoly complex (18-MPC) and Folin-Ciocalteu reagent (FCR) with a diversity of the representatives of different phenolic compounds classes shows a similar reaction behavior for both reagents. This evidence can be used for the application of 18-MPC along with FCR for the evaluation of total content of phenols. It was established that 18-MPC is more active as an oxidizing agent with respect to phenols and for this reason in comparison with FCR the range of optimal values of pH is displaced into the more acidic region, the reaction rate is higher, and the concentration of the reagent is one order of magnitude less. It was also discovered that by using both reagents and measuring the absorbance in absorption maximum of heteropoly blue (HPB), the graduation curves were nonlinear. The observed phenomenon was explained by the changes in the composition and depth of the HPB reduction occurring when changing the ratio between the heteropoly complex and the phenol. The isobestic point present in the absorption spectra of HPBs and which was acquired from 18-MPC allows obtaining strictly linear calibration curves. By using 18-MPC the influence of reducing sugars, simple monophenols, sulphite ions, vitamins and some other compounds on the result of the determination of phenolic compounds becomes much smaller. It was shown by example of the ascorbic acid and rutin determination in the mixture that only by using 18-MPC it was possible to determine phenol and more active reducing agent separately by varying the pH of the determination.

         Keywords: 18-molybdophosphate heteropoly complex, Folin-Ciocalteu reagent, spectrophotometric analysis, determination of phenols.


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Scalbert A., Williamson G. Dietary intake and bioavailability of polyphenols. J. Nutrition, 2000, vol. 130, no. 8, pp. 2073-2085.

Flavonoids: chemistry, biochemistry, and applications. O.M. Andersen, K.R. Markham (Ed.). Taylor&Francis, 2006. 1197 p.

Khimicheskii analiz lekarstvennykh rastenii [Chemical analysis of medicinal plants] Grinkevich N.I., Safronich L.N. (Ed.). Moscow, Vysshaia shkola, 1983. 176 p (in Russian).

Hollman P.C.H., Arts C.W. Flavonols, flavones and flavanols – nature, occurrence and dietary burden. J. Sci. Food Agric., 2000, vol. 80, no. 7, pp. 1081-1093. doi: 10.1002/(SICI)1097-0010(20000515)80:7<1081::AID-JSFA566>3.0.CO;2-G.

Khanbabaee K., T. van Ree. Tannins: Classification and definition. Nat. Prod. Rep., 2001, no. 18, pp. 641-649. doi: 10.1039/b101061l.

Weingerl W. A comparative study of analytical methods for determination of polyphenols in wine by HPLC/UV-Vis, spectrophotometry and chemiluminometry. In: Macro to nano spectroscopy. Ed. J. Uddin., Ch. 17. 2012, pp. 357-370.

Prior R.L., Wu X., Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem., 2005, vol. 53, no. 10, pp. 4290-4302. doi: 10.1021/jf0502698.

Khoddami A., Wilkes M.A., Roberts T.H. Techniques for analysis of plant phenolic compounds. Molecules, 2013. vol. 18, pp. 2328-2375. doi: 10.3390/molecules18022328.

Robards K., Antolovich M. Analytical chemistry of fruit bioflavonoids. A review. Analyst, 1997. vol. 122. pp. 11R-34R. doi: 10.1039/A606499J.

Ignat I., Volf I., Popa V.I. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem., 2011, vol. 126, no. 4,

pp. 1821-1835. doi: 10.1016/j.foodchem.2010.12.026.

Porgali E., Büyüktuncel E. Determination of phenolic composition and antioxidant capacity of native red wines by high performance liquid chromatography and spectrophotometric methods. Food Research International, 2012, vol. 45, no. 1,

pp. 145-154. doi: 10.1016/j.foodres.2011.10.025.

Escarpa A., González M.C. Approach to the content of total extractable phenolic compounds from different food samples by comparison of chromatographic and spectrophotometric methods. Anal. Chim. Acta, 2011, vol. 427, no. 1, pp. 119-127. doi: 10.1016/S0003-2670(00)01188-0.

Singleton V.L., Orthofer R., Lamuela-Raventos R.M. Analysis of total phenols and other oxidations substractes and antioxidans by means of Folin-Ciocalteu reagent. Methоds in enzymology, 1999, vol. 299, pp. 152-178. doi: 10.1016/S0076-6879(99)99017-1.

Jurd L. Aluminium complexes of phenolic flavones. Spectral and structural correlation. Phytochemistry, 1969, vol. 8, pp. 445-462. doi: 10.1016/S0031-9422(00)85447-3.

Özyrek M., Güçlü K., Tütem E., Başkan K.S., Erçağ E., Çelik S.E., Baki S., Yildiz L., Karaman Ş., Apak R. A comprehensive review of CUPRAC methodology. Anal. Meth., 2011, vol. 3, pp. 2439-2453. doi: 10.1039/C1AY05320E.

Benzie I.F.F., Strain J.J The ferric reducing ability of plasma (FRAP) as a measure of «antioxidant power»: The FRAP assay Anal. Biochem, 1996, vol. 239, no. 1,

pp.70-76. doi: 10.1006/abio.1996.0292.

Ainsworth E.A., Gillespie K.M. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols, 2007, vol. 2, no. 4, pp. 875-877. doi: 10.1038/nprot.2007.102.

Wu H. Contribution to the chemistry of phosphomolybdic acids, phosphotungstic acids, and allied substances. J. Biol. Chem., 1920, vol. 43, pp. 189-220.

Pop M.S. Geteropoli- i izopolioksometallaty [Heteropoly- and Isopolyoxometalates]. Novosibirsk, Nauka Publ., 1990. 227 p. (in Russian).

Contant R., Ciabrini J.-P. Stereospecific preparations of new n-molybdo-(18-n)-tungsto-2-phosphates and related “defect” compounds (n=2, 4 or 5). J. Inorg. Nucl. Chem., 1981, vol. 43, no. 7, pp. 1525-1528. doi: 10.1016/0022-1902(81)80330-2.

Briand L.E., Valle G.M., Thomas H.J. Stability of the phospho-molybdic Dawson-type ion P2Mo18O626- in aqueous media. J. Mater. Chem, 2002, vol. 12,

pp. 299-304. doi: 10.1039/b106769a.

Bulatov A.V., Petrova A.V., Vishnikin A.B., Moskvin A.L., Moskvin L.N. Stepwise injection spectrophotometric determination of epinephrine. Talanta, 2012, vol. 96,

pp. 62-67. doi: 10.1016/j.talanta.2012.03.059.

Al-Shwaiyat M.K.E.A., Denisenko T. A., Zaruba S.V.,Vishnikin A.B., Tsiganok L.P., Andruch V., Bazel Y.R. Simultaneous determination of two active components of pharmaceutical preparations by sequential injection method using heteropoly complexes. Vestnik Dnepropetrovskogo natsional'nogo universiteta. Seriia khimicheskaia [Bulletin of Dnipropetrovsk National University. Series Chemistry], 2014, vol. 22. no. 1. pp. 16-22. doi: 10.15421/081408.

Vishnikin A.B., Al-Shwaiyat M.K.E.A., Petrushina G.A., Tsiganok L.P., Andruch V., Bazelc Y.R., Sklenařova H., Solich P. Highly sensitive sequential injection determination of p-aminophenol in paracetamol formulations with

-molybdodiphosphate heteropoly anion based on elimination of Schlieren effect. Talanta, 2012, vol. 96, pp. 230-235(in Russian). doi: 10.1016/j.talanta.2012.02.049.

Vishnikin A.B., Sklenařova H., Solich P., Petrushina G.A., Tsiganok L.P. Determination of ascorbic acid with Wells-Dawson type molybdophosphate in sequential injection system. Anal. Lett. 2011, vol. 44, no. 1-3, pp. 514-527. doi: 10.1080/00032719.2010.500789.

Denisenko T.A., Tsyganok L.P., Vishnikin A.B. [Spectrophotometric determination of quercetin and sum of polyphenols with 18-molybdodiphosphoric heteropoly complex]. Metody i ob``ekty khimicheskogo analiza [Methods and objects of chemical analysis], 2014, vol. 9, no. 3, pp. 130-138 (in Russian).

Georgé S., Brat P., Alter P., Amiot M.J. Rapid determination of polyphenols and vitamin C in plant-derived products. J. Agric. Food Chem., 2005, vol. 53, no. 5,

pp. 1370-1373. doi: 10.1021/jf048396b.


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