АМПЕРОМЕТРИЧЕСКИЕ ИММУНОСЕНСОРЫ НА ОСНОВЕ УГЛЕРОДНЫХ НАНОМАТЕРИАЛОВ И КЛАСТЕРОВ РЕНИЯ ДЛЯ ОПРЕДЕЛЕНИЯ ТРИЦИКЛИЧЕСКИХ АНТИДЕПРЕССАНТОВ В ПОСЛЕДОВАТЕЛЬНОЙ ИНЖЕКЦИОННОЙ СИСТЕМЕ
Аннотация
Предложен способ последовательного инжекционного определения трициклических антидепрессантов (амитриптилин, дезипрамин, имипрамин) с использованием амперометрических иммуносенсоров на основе печатных графитовых электродов, модифицированных углеродными наноматериалами (углеродные нанотрубки, оксид графена или фуллерен) в сочетании с гексарениевыми халькогенидными кластерами. Последовательный инжекционный анализ проводили при скорости развертки 10 мВ/с, также варьировали скорость потока (фоновый электролит - фосфатный буферный раствор с рН 7.0) от 20 до 40 мкл/с. Состав модификатора оказывает влияние на аналитические возможности иммуносенсоров. Скрининг различных вариантов модификаторов показал, что наибольшая чувствительность 23.9 ± 0.9 с диапазоном рабочих концентраций 1 ·10-10 – 1 · 10-5 М характерна для иммуносенсора на основе оксида графена и цианидных комплексов халькогенидных кластеров рения, а более широкая область определяемых содержаний 1 · 10-10 – 1 · 10-4 М наблюдается в случае иммуносенсора на основе фуллерена и цианидных комплексов халькогенидных кластеров рения. Нижняя граница определяемых содержаний находится на уровне (4 – 7)·10-11 М.
Относительное стандартное отклонение повторяемости (Sr) не более 0.046. Полученные результаты показывают, что предлагаемые иммуносенсоры могут найти применение в последовательном инжекционном определении следовых содержаний лекарственных соединений (антидепрессантов) в медико-биологических объектах.
Ключевые слова: иммуносенсор, углеродные наноматериалы, гексарениевые халькогенидные кластеры, трициклические антидепрессанты, последовательный инжекционный анализ
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DOI: https://doi.org/10.15826/analitika.2022.26.4.002
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